Subtropical and ornamental horticulture
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TEA CULTURE IN DEPOSIT IN VITRO-COLLECTION OF THE RUSSIAN RESEARCH INSTITUTE OF FLORICULTURE AND SUBTROPICAL CROPS

Year:
2019
The authors:
Gvasaliya M. V.
DOI:
10.31360/2225-3068-2019-68-119-125
Page numbers:
119 - 125
Keywords:
tea biotechnology, deposition, in-vitro transplantation of tea, gene pool

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Russian Research Institute of Floriculture and Subtropical Crops is the only in Russia holder of tea culture collection in vitro. At the deposit tea plant microshoots are in 2 cultivation modes: in transplantation culture – on nutrient medium Murashige and Skoog (MS) + 6 – benzylaminopurine (BAP) – 2.0 + naphthylacetic acid – 0.2 + gibberellic acid (GА) – 1.5 ml + mesoinosit – 100 mg/l; and in medium-term storage, with a suspension of growth (for 12 months), in the conditions of the refrigerating chamber, at ½ of the nonhormonal nutrient medium MS, at a temperature of + 8 о C, illumination of 800 lux, light mode 16/8 h. Grown in laboratory conditions tea microplants serve as the source material for micro-multiplication, exchange of the gene pool, and breeding studies.
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ISSN 2225-3068

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