Subtropical and ornamental horticulture
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IN VITRO-CULTURE INITIATION AND MICROPROPAGATION OF FEIJOA CULTIVAR ‘COOLIDGE’

Year:
2019
The authors:
Kastritskaya M. S., Kukharchik N. V.
DOI:
10.31360/2225-3068-2019-68-125-131
Page numbers:
125 - 131
Keywords:
feijoa, culture in vitro, micropropagation, rhizogenesis, adaptation, Belarus

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The paper studied one of feijoa propagation methods – in vitro micropropagation. The study results on in vitro culture initiation of feijoa cultivar ‘Coolidge’ are presented, high efficiency of feijoa in vitro initiation using vegetative buds is shown. We determined the sterilization method and optimal nutrient medium for the initiation stage – Murashige Skoog (MS) supplemented with 100 mg/l of mezo-inositol, 1.0 mg/l of biotin, 1.0 mg/l of folic acid, and 2.0 mg/l of cytokinin 2-iP. Optimal media on the base of Woody Plant Medium (WPM) with phytohormones (zeatine, 2-isopentenyladenine) are described for the micropropagation stage with the multiplication coefficient 1.8–3.0. We also determined the concentration of IBA and composition of the nutrient medium at the stage of rhizogenesis, which allowed us within 2 months to get rooted regenerants of 2.9 cm long having 3 internodes, 2.4 adventitious roots, at least 6.8 cm long. Our studies on feijoa adaptation showed a high rate of survived regenerants on two examined substrates: 65 % on BIONA-112 and 89 % on peat substrate (‘Dvina’ peat + perlite).
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ISSN 2225-3068

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