Subtropical and ornamental horticulture
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INTRODUCTION OF OSMUNDA REGALIS L. TO IN VITRO

Year:
2020
The authors:
Malyarovskaya V. I., Rakhmangulov R. S., Koninskaya N. G.
DOI:
10.31360/2225-3068-2020-72-86-93
Page numbers:
86 - 93
Keywords:
Osmunda regalis, fern, in vitro culture, gametophyte, sterilization, nutrient media

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The paper studied the introduction of an endangered, rare fern Osmunda regalis L. into sterile culture. It was found that within the introduction of O. regalis sporangia to tissue culture the most optimal sterilizing agents were 2% hydrogen peroxide in the 20-minute exposure (54.1 % of viable spores) and 5 % sodium hypochlorite in the 20-minute exposure (48.4 % of viable spores). It was revealed that the best nutrient medium for micro-propagation of fern gametophytes contains ½ of the micro and macrosols composition according to Murashige-Skoog (MS) without ammonium nitrate and vitamins.
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