MICROPROPAGATION OF REMONTANT RASPBERRY CULTIVARS AND HYBRID
- The authors:
- Gashenko O. A., Kukharchik N. V., Frolova L. V.
- Page numbers:
- 112 - 119
- raspberry, in vitro culture, micropropagation, explant, Rubus L., Belarus.
The research was conducted in the Department of Biotechnology (Republican Research and Production Unitary Enterprise “Institute of Fruit Growing”) in 2019. The objects of research were 3 samples of remontant raspberry (cv. ‘Gerakl’ and cv. ‘Heritage’, domestic hybrid 02-03-10). The paper evaluated an effectiveness of in vitro initiation of raspberry cultivars and the hybrid. The effectiveness in vitro initiation ranged from 72 to 92.6 %. For cv. ‘Heritage’ and hybrid 02-03-10, the best results of in vitro culture initiation were observed in the second ten-day festival of November and in the second ten-day festival of January (the number of infected explants did not exceed 5 %, necrotic – 14.6 %). For cv. ‘Gerakl’, all studied terms of in vitro culture initiation were acceptable. The parameters of micropropagation of raspberry cultivars and the hybrid were established. At micropropagation stage (medium with addition of 0.8 mg/l 6-BA), best propagation rates were noted for cv. ‘Gerakl’ – up to 3.57, then for hybrid 02-03-10 – up to 1.73 and for cv. ‘Heritage’ – up to 1.13. The number of subcultures did not have a significant effect (p > 0.5) on the length of microplants studied.