PHENOTYPE AND GENOTYPE VARIABILITY OF TEA (CAMELLIA SINENSIS (L.) KUNTZE) SOMATIC CLONES IN VITRO
- The authors:
- Gvasaliya M. V.
- Page numbers:
- 111 - 119
- (Camellia sinensis (L.) Kuntze), somatic clones, phenotype, morphological characteristics, genetic variability in vitro, genome size.
Russian scientists have studied in detail the positive and negative signs of the leaf, which directly correlate with the yield and biochemical parameters. There is a wide range of morphological features, according to which it is recommended to select valuable forms of tea for breeding. The conducted studies revealed in vitro variability at the phenotype level in somatic tea clones obtained through callus culture. The mutations affected the main morphological characteristics of the leaf: its size, shape, surface, and colour. Almost all somaclones differed both among themselves and from with the original phenotype – the local population. According to the complex of positive signs, 6 promising forms were recorded, which can be used in various breeding programs. Flow cytometry (Beckman Coulter cytometer) was used to analyze the genome size of tea somaclones (Sc –11; Sc –27; Sc –33) isolated by phenotype, and their variability was confirmed at the genetic level.