THE EFFECT OF NUTRIENT MEDIAS WITH DIFFERENT HORMONAL COMPOSITION ON THE INTENSITY OF PROPAGATION OF CHERRY CULTIVARS IN VITRO
- The authors:
- Tashmatova L. V., Ryago N. V., Melyanovskaya A. Y.
- Page numbers:
- 76 - 82
- cherry, reproduction, in vitro, viability, nutrient medium, growth stimulants.
In order to improve the classical breeding process, biotechnological methods and techniques, in particular, in vitro cultivation, used with the aim to reduce the time of obtaining valuable genotypes, are becoming increasingly important. The paper presents research results about the effect of nutrient medias with different hormonal composition on the intensity of propagation of cherry cultivars in vitro. The experiment was conducted in Orel, on the basis of the Biotechnology Laboratory (Russian Research Institute for Fruit Crops Breeding), in 2019 and 2020. The study objects were the cultivars: ‘Businka’, ‘Bystrinka’, ‘Kapelka’, ‘Livenskaya’, ‘Mtsenskaya’, ‘Novella’, ‘Orlitsa’, ‘Podarok uchitelyam’, ‘Prevoskhodnaya Kolesnikovoy’, ‘Putinka’, ‘Rovesnitsa’, ‘Turgenevka’, ‘Shocoladnitsa’. At the stage of reproduction in vitro, mineral bases from Murashige and Skoog medium (MS) and Woody Plant Medium (WPM) (Lloyd and McCown medium) were used. Analysis of the data obtained allowed us to conclude that the optimal medium for cultivating cherry cultivars at the proliferation stage is a medium based on mineral salts from WPM.