MICROSATELLITE ANALYSIS OF ANNUAL CROP SAMPLES IN VITRO AND INITIAL TREES FOR CLONAL MICROPROPAGATION OF QUERCUS ROBUR L. TRESS
- The authors:
- Rzhevsky S. G., Guseva O. Y.
- Page numbers:
- 90 - 95
- oak, microsatellites, SSR, in vitro culture, genetic certification.
This paper presents the result of microsatellite analysis of 40-year-old oak tree samples (Quercus robur L.), which are a breeding object of the Semiluksky nursery (Voronezh region). The work used the material of 40-year-old trees, as well as microclones obtained from them in vitro. The analysis was carried out on six SSR loci, which had previously proven themselves well for the genetic certification of oak. As a result of the study, the identity of the genetic structure of microsatellite loci of the original oak samples and microclones produced from them, within the resolution of the method, was revealed. These data support the resistance of genotype structures during their in vitro cultivation, which is important in preserving economically valuable plant traits.