СHARACTERISTIC OF OBTAINING PRIMARY MORPHOGENIC CULTURES IN VITRO FROM 40-YEAR-OLD OAK TRESS
- The authors:
- Guseva O. Y.
- Page numbers:
- 85 - 89
- Quercus robur L., asepsis, in vitro, nodal segments, explants.
The method of clonal micropropagation used in order to preserve economically valuable specimens of common oak may be limited due to the strong infection of plant material from adult trees. This paper studied the effect of sterilization regime and genotype on the production of viable oak cultures. Green shoots from 40-year-old oak trees were selected for the work during an active growing season. Treatment with merthiolate allowed us to obtain a high percentage of aseptic crops in 2020, while the bleach solution turned out to be unsuitable for sterilization of summer oak shoots. The success of asepsis was also influenced by the coefficient of genotypic variation in initial trees, which was partially balanced by using a more rigid sterilizing agent. In general, the effectiveness of asepsis in 2020 was higher compared to the results obtained in the following year. This phenomenon may be associated with abnormally hot weather in the Voronezh region during harvest of samples for further research in the summer of 2021.